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CCDC9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC9 antibodies →
$504.00each
Catalog Number: LS-G113120-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC9 (NM_015603), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC9 | DKFZP586M1019
Origin Species
Human
NCBI GenBank
NM_015603
SwissProt
Q9Y3X0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAATLDLKSK EEKDAELDKR IEALRRKNEA LIRRYQEIEE DRKKAELEGV AVTAPRKGRS
61VEKENVAVES EKNLGPSRRS PGTPRPPGAS KGGRTPPQQG GRAGMGRASR SWEGSPGEQP
121RGGGAGGRGR RGRGRGSPHL SGAGDTSISD RKSKEWEERR RQNIEKMNEE MEKIAEYERN
181QREGVLEPNP VRNFLDDPRR RSGPLEESER DRREESRRHG RNWGGPDFER VRCGLEHERQ
241GRRAGLGSAG DMTLSMTGRE RSEYLRWKQE REKIDQERLQ RHRKPTGQWR REWDAEKTDG
301MFKDGPVPAH EPSHRYDDQA WARPPKPPTF GEFLSQHKAE ASSRRRRKSS RPQAKAAPRA
361YSDHDDRWET KEGAASPAPE TPQPTSPETS PKETPMQPPE IPAPAHRPPE DEGEENEGEE
421DEEWEDISED EEEEEIEVEE GDEEEPAQDH QAPEAAPTGI PCSEQAHGVP FSPEEPLLEP
481QAPGTPSSPF SPPSGHQPVS DWGEEVELNS PRTTHLAGAL SPGEAWPFES VTRTRPLEQK
541LISEEDLAAN DILDYKDDDD KV

562 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.