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CCDC88C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC88C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC88C antibodies →
$725.00each
Catalog Number: LS-G120201-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC88C (NM_001080414), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
228.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDVTVSELLE LFLQSPLVTW VKTFGPFGSG SQDNLTMYMD LVDGIFLNQI MLQIDPRPTN
61QRINKHVNND VNLRIQNLTI LVRNIKTYYQ EVLQQLIVMN LPNVLMIGRD PLSGKSMEEI
121KKVLLLVLGC AVQCERKEEF IERIKQLDIE TQAGIVAHIQ EVTHNQENVF DLQWLELPDV
181APEELEALSR SMVLHLRRLI DQRDECTELI VDLTQERDYL QAQHPPSPIK SSSADSTPSP
241TSSLSSEDKQ HLAVELADTK ARLRRVRQEL EDKTEQLVDT RHEVDQLVLE LQKVKQENIQ
301LAADARSARA YRDELDSLRE KANRVERLEL ELTRCKEKLH DVDFYKARME ELREDNIILI
361ETKAMLEEQL TAARARGDKV HELEKENLQL KSKLHDLELD RDTDKKRIEE LLEENMVLEI
421AQKQSMNESA HLGWELEQLS KNADLSDASR KSFVFELNEC ASSRILKLEK ENQSLQSTIQ
481GLRDASLVLE ESGLKCGELE KENHQLSKKA GSLHLQIEKL QTQLEREKQS NQDLETLSEE
541LIREKEQLQS DMETLKADKA RQIKDLEQEK DHLNRAMWSL RERSQVSSEA RMKDVEKENK
601ALHQTVTEAN GKLSQLEFEK RQLHRDLEQA KEKGERAEKL ERELQRLQEE NGRLARKVTS
661LETATEKVEA LEHESQGLQL ENRTLRKSLD TLQNVSLQLE GLERDNKQLD AENLELRRLV
721ETMRFTSTKL AQMERENQQL EREKEELRKN VDLLKALGKK SERLELSYQS VSAENLRLQQ
781SLESSSHKTQ TLESELGELE AERQALRRDL EALRLANAQL EGAEKDRKAL EQEVAQLEKD
841KKLLEKEAKR LWQQVELKDA VLDDSTAKLS AVEKESRALD KELARCRDAA GKLKELEKDN
901RDLTKQVTVH ARTLTTLRED LVLEKLKSQQ LSSELDKLSQ ELEKVGLNRE LLLQEDDSGS
961DTKYKILEGR NESALKTTLA MKEEKIVLLE AQMEEKASLN RQLESELQML KKECETLRQN
1021QGEGQHLQNS FKHPAGKTAA SHQGKEAWGP GHKEATMELL RVKDRAIELE RNNAALQAEK
1081QLLKEQLQHL ETQNVTFSSQ ILTLQKQSAF LQEHNTTLQT QTAKLQVENS TLSSQSAALT
1141AQYTLLQNHH TAKETENESL QRQQEQLTAA YEALLQDHEH LGTLHERQSA EYEALIRQHS
1201CLKTLHRNLE LEHKELGERH GDMLKRKAEL EEREKVLTTE REALQQEQRT NALAMGENQR
1261LRGELDRVNF LHHQLKGEYE ELHAHTKELK TSLNNAQLEL NRWQARFDEL KEQHQTMDIS
1321LTKLDNHCEL LSRLKGNLEE ENHHLLSQIQ LLSQQNQMLL EQNMENKEQY HEEQKQYIDK
1381LNALRRHKEK LEEKIMDQYK FYDPPPKKKN HWIGAKALVK LIKPKKEGSR ERLKSTVDSP
1441PWQLESSDPA SPAASQPLRS QAENPDTPAL GSNCAEERDA HNGSVGKGPG DLKPKRGSPH
1501RGSLDRTDAS TDLAMRSWPS ELGSRTCSTS ATTTAPSNST PIARHPGRTK GYNSDDNLCE
1561PSLEFEVPNH RQYVSRPSSL ESSRNTSSNS SPLNLKGSSE QLHGRSESFS SEDLIPSRDL
1621ATLPREASTP GRNALGRHEY PLPRNGPLPQ EGAQKRGTAP PYVGVRPCSA SPSSEMVTLE
1681EFLEESNRSS PTHDTPSCRD DLLSDYFRKA SDPPAIGGQP GPPAKKEGAK MPTNFVAPTV
1741KMAAPTSEGR PLKPGQYVKP NFRLTEAEAP PSVAPRQAQP PQSLSLGRPR QAPVPPASHA
1801PASRSASLSR AFSLASADLL RASGPEACKQ ESPQKLGAPE ALGGRETGSH TLQSPAPPSS
1861HSLARERTPL VGKAGSSCQG PGPRSRPLDT RRFSLAPPKE ERLAPLHQSA TAPAIATAGA
1921GAAAAGSGSN SQLLHFSPAA APAARTKPKA PPRSGEVATI TPVRAGLSLS EGDGVPGQGC
1981SEGLPAKSPG RSPDLAPHLG RALEDCSRGS VSKSSPASPE PGGDPQTVWY EYGCV
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

2035 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.