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CCDC87 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC87 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC87 antibodies →
$504.00each
Catalog Number: LS-G111877-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC87 (NM_018219), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC87
Origin Species
Human
NCBI GenBank
NM_018219
SwissProt
Q9NVE4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MMEPPKPEPE LQRFYHRLLR PLSLFPTRTT SPEPQKRPPQ EGRILQSFPL AKLTVASLCS
61QVAKLLAGSG IAAGVPPEAR LRLIKVILDE LKCSWREPPA ELSLSHKNNQ KLRKRLEAYV
121LLSSEQLFLR YLHLLVTMST PRGVFTESAT LTRLAASLAR DCTLFLTSPN VYRGLLADFQ
181ALLRAEQASG DVDKLHPVCP AGTFKLCPIP WPHSTGLAQV QCSNLNLNYL IQLSRPPEFL
241NEPGRMDPVK ELKSIPRLKR KKPFHWLPSI GKKREIDISS SQMVSLPSYP VAPTSRASPS
301PFCPELRRGQ SMPSLREGWR LADELGLPPL PSRPLTPLVL ATESKPELTG LIVAEDLKQL
361IKKMKLEGTR YPPLDSGLPP LLGVVTRHPA AGHRLEELEK MLRNLQEEEA SGQWDPQPPK
421SFPLHPQPVT ITLKLRNEVV VQAAAVRVSD RNFLDSFHIE GAGALYNHLA GELDPKAIEK
481MDIDNFVGST TREVYKELMS HVSSDHLHFD QGPLVEPAAD KDWSTFLSSA FLRQEKQPQI
541INPELVGLYS QRANTLQSNT KKMPSLPSLQ ATKSWEKWSN KASLMNSWKT TLSVDDYFKY
601LTNHETDFLH VIFQMHEEEV PVEIVAPARE SLEIQHPPPL LEDEEPDFVP GEWDWNTVLE
661HRLGAGKTPH LGEPHKILSL QKHLEQLWSV LEVPDKDQVD MTIKYSSKAR LRQLPSLVNA
721WERALKPIQL REALLARLEW FEGQASNPNR FFKKTNLSSS HFLEENQVRS HLHRKLNLME
781SSLVSLLEEI ELIFGEPVIF KGRPYLDKMK SDKVEMLYWL QQQRRVRHLV SALKDPHQST
841LFRSSAASL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

849 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.