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CCDC85A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC85A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC85A antibodies →
$725.00each
Catalog Number: LS-G120219-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC85A (NM_001080433), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC85A | KIAA1912
Origin Species
Human
SwissProt
Q96PX6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSKAAGGAAA AAAAAESCSP APAGSSAAPP APVEDLSKVS DEELLQWSKE ELIRSLRRAE
61AEKVSAMLDH SNLIREVNRR LQLHLGEIRG LKDINQKLQE DNQELRDLCC FLDDDRQKGK
121RVSREWQRLG RYTAGVMHKE VALYLQKLKD LEVKQEEVVK ENMELKELCV LLDEEKGAGC
181AGSRCSIDSQ ASLCQLTAST APYVRDVGDG SSTSSTGSTD SPDHHKHHAS SGSPEHLQKP
241RSEGSPEHSK HRSASPEHPQ KPRACGTPDR PKALKGPSPE HHKPLCKGSP EQQRHPHPGS
301SPETLPKHVL SGSPEHFQKH RSGSSPEHAR HSGGSPEHLQ KHALGGSLEH LPRARGTSPE
361HLKQHYGGSP DHKHGGGSGG SGGSGGGSRE GTLRRQAQED GSPHHRNVYS GMNESTLSYV
421RQLEARVRQL EEENRMLPQA SQNRRQPPTR NSSNMEKGWG SRARRVLQWW QGCRGIGRCL
481PTLPGSFRLS SGADGSNSSP NSAASFSGHA TPSQQPEPVV HSLKVVWRKL GDAAGSCPGI
541RQHLSGNQYK GPM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

553 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.