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CCDC82 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC82 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC82 antibodies →
$504.00each
Catalog Number: LS-G109812-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC82 (NM_024725), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC82 | HSPC048
Origin Species
Human
NCBI GenBank
NM_024725
SwissProt
Q8N4S0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIHVRRHETR RNSKSHVPEQ KSRVDWRRTK RSSISQLLDS DEELDSEEFD SDEELDSDES
61FENDEELDSN KGPDCNKTPG SERELNLSKI QSEGNDSKCL INSGNGSTYE EETNKIKHRN
121IDLQDQEKHL SQEDNDLNKQ TGQIIEDDQE KHLSQEDNDL NKQTGQIIED DLEEEDIKRG
181KRKRLSSVMC DSDESDDSDI LVRKVGVKRP RRVVEDEGSS VEMEQKTPEK TLAAQKREKL
241QKLKELSKQR SRQRRSSGRD FEDSEKESCP SSDEVDEEEE EDNYESDEDG DDYIIDDFVV
301QDEEGDEENK NQQGEKLTTS QLKLVKRNSL YSFSDHYTHF ERVVKALLIN ALDESFLGTL
361YDGTRQKSYA KDMLTSLHYL DNRFVQPRLE SLVSRSRWKE QYKERVENYS NVSIHLKNPE
421NCSCQACGLH RYCKYSVHLS GELYNTRTMQ IDNFMSHDKQ VFTVGRICAS RTRIYHKLKH
481FKFKLYQECC TIAMTEEVED EQVKETVERI FRRSKENGWI KEKYGQLEEY LNFADYFQEE
541KFEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

544 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.