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CCDC81 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC81 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC81 antibodies →
$725.00each
Catalog Number: LS-G110596-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC81 (NM_021827), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC81
Origin Species
Human
NCBI GenBank
NM_021827
SwissProt
Q6ZN84

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLDTIARALQ DLGRQVLPTL PSLSQEEVSI IWGNVSEFVR RQLTLHKGVQ IPAFGTFTFI
61RQKLEVGNNK FILIQRPVFI MVEKLVQIHG LKQNKRPGTV DSVLSSREAL RKWPSSVLAF
121PRIELKEMEN KLPMETLVEE CGENRERKCK LKDQSDKEEG TRDISSPKRL RDRQALFPAK
181VTNVSLLEKF ERSESGGKIM TPESLSYPSC LKHDSEMKPQ TSPACQDHNK AGQEMCYVCL
241QRAQRNSLLY YSEERRREIE DERLIQQYQM LKDQEALFRH QMKSLATREQ NQKNAAYNLG
301VAEAIRNHKN EKPEFYKSFL FDKRPLSPAL NALKQEEYSR SLLKQMDNRQ ENEIKQRQYR
361ELMDRLEQVQ LTEELAAQRA KFLKDKMEET QCYKRALDAQ IKNKPSRLPP FEPDSSEPIF
421GKNEGELMVE KQKREQNYMK HQLEAAANHK RKAILHQLVD QRRDLQMLQR TQREHLADRT
481AELERVNRVN QCLQEDWERS AAMKKQRDLE DKAFERASDK LFLLDQCEKY RRCKQCQRRT
541SNVGESNLWP LNKFLPGSRL LV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

562 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.