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CCDC80 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC80 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC80 antibodies →
$725.00each
Catalog Number: LS-G103354-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC80 (NM_199512), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC80 | DRO1 | SSG1 | Steroid sensitive gene 1 | URB | Okuribin
Origin Species
Human
SwissProt
Q76M96

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
108 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTWRMGPRFT MLLAMWLVCG SEPHPHATIR GSHGGRKVPL VSPDSSRPAR FLRHTGRSRG
61IERSTLEEPN LQPLQRRRSV PVLRLARPTE PPARSDINGA AVRPEQRPAA RGSPREMIRD
121EGSSARSRML RFPSGSSSPN ILASFAGKNR VWVISAPHAS EGYYRLMMSL LKDDVYCELA
181ERHIQQIVLF HQAGEEGGKV RRITSEGQIL EQPLDPSLIP KLMSFLKLEK GKFGMVLLKK
241TLQVEERYPY PVRLEAMYEV IDQGPIRRIE KIRQKGFVQK CKASGVEGQV VAEGNDGGGG
301AGRPSLGSEK KKEDPRRAQV PPTRESRVKV LRKLAATAPA LPQPPSTPRA TTLPPAPATT
361VTRSTSRAVT VAARPMTTTA FPTTQRPWTP SPSHRPPTTT EVITARRPSV SENLYPPSRK
421DQHRERPQTT RRPSKATSLE SFTNAPPTTI SEPSTRAAGP GRFRDNRMDR REHGHRDPNV
481VPGPPKPAKE KPPKKKAQDK ILSNEYEEKY DLSRPTASQL EDELQVGNVP LKKAKESKKH
541EKLEKPEKEK KKKMKNENAD KLLKSEKQMK KSEKKSKQEK EKSKKKKGGK TEQDGYQKPT
601NKHFTQSPKK SVADLLGSFE GKRRLLLITA PKAENNMYVQ QRDEYLESFC KMATRKISVI
661TIFGPVNNST MKIDHFQLDN EKPMRVVDDE DLVDQRLISE LRKEYGMTYN DFFMVLTDVD
721LRVKQYYEVP ITMKSVFDLI DTFQSRIKDM EKQKKEGIVC KEDKKQSLEN FLSRFRWRRR
781LLVISAPNDE DWAYSQQLSA LSGQACNFGL RHITILKLLG VGEEVGGVLE LFPINGSSVV
841EREDVPAHLV KDIRNYFQVS PEYFSMLLVG KDGNVKSWYP SPMWSMVIVY DLIDSMQLRR
901QEMAIQQSLG MRCPEDEYAG YGYHSYHQGY QDGYQDDYRH HESYHHGYPY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

950 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.