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CCDC74A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC74A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC74A antibodies →
$504.00each
Catalog Number: LS-G107220-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC74A (NM_138770), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC74A | CCDC74AV3 | CCDC74AV2
Origin Species
Human
NCBI GenBank
N45040
SwissProt
Q96AQ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
41.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MSGAGVAAGT RPPSSPTPGS RRRRQRPSVG VQSLRPQSPQ LRQSDPQKRN LDLEKSLQFL
61QQQHSEMLAK LHEEIEHLKR ENKDLHYKLI MNQTSQKKDG PSGNHLSRAS APLGARWVCI
121NGVWVEPGGP SPARLKEGSS RTHRPGGKRG RLAGGSADTV RSPADSLSMS SFQSVKSISN
181SGKARPQPGS FNKQDSKADV SQKADLEEEP LLHNSKLDKV PGVQGQARKE KAEASNAGAA
241CMGNSQHQGR QMGAGAHPPM ILPLPLRKPT TLRQCEVLIR ELWNTNLLQT QELRHLKSLL
301EGSQRPQAAP EEASFPRDQE ATHFPKVSTK SLSKKCLSPP VAERAILPAL KQTPKNNFAE
361RQKRLQAMQK RRLHRSVLTR TRPLEQKLIS EEDLAANDIL DYKDDDDKV

409 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.