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CCDC67 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC67 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC67 antibodies →
$725.00each
Catalog Number: LS-G104486-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC67(CCDC67) (NM_181645), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC67
Origin Species
Human
NCBI GenBank
AI199391
SwissProt
Q05D60

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENQAHNTMG TSPCEAELQE LMEQIDIMVS NKKMDWERKM RALETRLDLR DQELANAQTC
61LDQKGQEVGL LRQKLDSLEK CNLAMTQNYE GQLQSLKAQF SKLTNNFEKL RLHQMKQNKV
121PRKELPHLKE EIPFELSNLN QKLEEFRAKS REWDKQEILY QTHLISLDAQ QKLLSEKCNQ
181FQKQAQSYQT QLNGKKQCLE DSSSEIPRLI CDPDPNCEIN ERDEFIIEKL KSAVNEIALS
241RNKLQDENQK LLQELKMYQR QCQAMEAGLS EVKSELQSRD DLLRIIEMER LQLHRELLKI
301GECQNAQGNK TRLESSYLPS IKEPERKIKE LFSVMQDQPN HEKELNKIRS QLQQVEEYHN
361SEQERMRNEI SDLTEELHQK EITIATVTKK AALLEKQLKM ELEIKEKMLA KQKVSDMKYK
421AVRTENTHLK GMMGDLDPGE YMSMDFTNRE QSRHTSINKL QYENERLRND LAKLHVNGKS
481TWTNQNTYEE TGRYAYQSQI KVEQNEERLS HDCEPNRSTM PPLPPSTFQA KEMTSPLVSD
541DDVFPLSPPD MSFPASLAAQ HFLLEEEKRA KELEKLLNTH IDELQRHTEF TLNKYSKLKQ
601NRHI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

604 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.