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CCDC66 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC66 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC66 antibodies →
$725.00each
Catalog Number: LS-G125822-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC66 (NM_001141947), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC66
Origin Species
Human
SwissProt
A2RUB6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNLGDGLKLE TELLDGKTKL ILSPYEHKSK ISVKMGNKAK IAKCPLRTKT GHILKSTQDT
61CIGSEKLLQK KPVGSETSQA KGEKNGMTFS STKDLCKQCI DKDCLHIQKE ISPATPNMQK
121TRNTVNTSLV GKQKPHKKHI TAENMKSSLV CLTQDQLQQI LMTVNQGNRS LSLTENGKEA
181KSQYSLYLNS ISNQPKDENI MGLFKKTEMV SSVPAENKSV LNEHQETSKQ CEQKIAIENE
241WKPADIFSTL GERECDRSSL EAKKAQWRKE LDEQVALKKK EKEVSEKWND PWKKSESDKI
301IWEKHQILDQ SRETVLLEHP FSAVKQELQR KWIEELNKQI EDDRQRKIEE KIIYSKGEEH
361DRWAMHFDSL KSYPGSQSQL FSQSTHKQPE YFCVSPDTQE LADVSSVCTP TTGSQVEPSE
421EEHIAKPIKD VVMANSKKTN FLRSMTALLD PAQIEERDRR RQKQLEHQKA ITAQVEEKRR
481KKQLEEEQRK KEEQEEELRL AQEREEMQKQ YEEDILKQKQ KEEIMTLKTN ELFQTMQRAQ
541ELAQRLKQEQ RIRELAQKGH DTSRLIKNLG VDTIQMEYNA SNISNSRHDS DEISGKMNTY
601MNSTTSKKDT GVQTDDLNIG IFTNAESHCG SLMERDITNC SSPEISAELI GQFSTKKNKQ
661ELTQDKGASL EKENNRCNDQ CNQFTRIEKQ TKHMKKYPKR PDWNINKPPK RYIPASEKYP
721KQLQKQREEK KVRRQMELLH LVEKNNPGHL SQNRGISPEI FHSSHQETES KLRWHLVKKE
781EEPLNIHSFS KERSPSSPVP VVKNRTQQTQ NTLHLPLKNS SYERENLISG SNQTELSSGI
841SESSHFIPYV RTNEIYYLDP DAPLSGPSTQ DPQYQNSQDC GQKRQLFDSD CVRDPLLNPN
901MVKNRDRQQA ILKGLSELRQ GLLQKQKELE SSLLPLAENQ EESFGSSF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

948 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.