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CCDC65 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC65 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108427-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC65 (NM_033124), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_033124

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MPKKEKMAKT PLSDEKQLLL FQQKLLAEEE MAKKKERLLS QFLKDKLAKE EHNSALNLNK
61INTQWRTVLR EVKTRELHKD IEILSQTFER VVDCKDNVIK SLAKDLSEAE EQYAHALRSH
121LHNVDQLLAL QRHRLSLLEE SYNMELEALT KEFETERKTI IDQHEKEIHY LQDIFMAMEQ
181NYIDSEYESK LEFQSMWNDL KNMNLEEKHF LRLHLENRVE DLWRKFQDVL KNYTDASEDR
241KAAFETLQVK DEKSSKEIEV QMKKIQKLQD AITISKGKIM IHSRESEDEN RYIRNDKELV
301LVQLRKLKAQ RTQARAASQK NLVRLTLESN ATLKALRKIV DKGEKILKLA EICRKFETEE
361EKVLPFYSSV LTPKEQEGIQ KNNLEELTEE LTKVMVDYIG MENFWKRYNK VKLEQLSLQH
421RRAQLLDING KLREMLKQYL DGISVSDEVL SQLNPLFIVN YQSNLLQPLS IRIAHPGDKQ
481HPTT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

484 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.