LSBio
CCDC64B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC64B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC64B antibodies →
$725.00each
Catalog Number: LS-G118850-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC64B(CCDC64B) (NM_001103175), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSPDGPSFP SGPLSGGASP SGDEGFFPFV LERRDSFLGG GPGPEEPEDL ALQLQQKEKD
61LLLAAELGKM LLERNEELRR QLETLSAQHL EREERLQQEN HELRRGLAAR GAEWEARAVE
121LEGDVEALRA QLGEQRSEQQ DSGRERARAL SELSEQNLRL SQQLAQASQT EQELQRELDA
181LRGQCQAQAL AGAELRTRLE SLQGENQMLQ SRRQDLEAQI RGLREEVEKG EGRLQTTHEE
241LLLLRRERRE HSLELERARS EAGEALSALR RLQRRVSELE EESRLQDADV SAASLQSELA
301HSLDDGDQGQ GADAPGDTPT TRSPKTRKAS SPQPSPPEEI LEPPKKRTSL SPAEILEEKE
361VEVAKLQDEI SLQQAELQSL REELQRQKEL RAQEDPGEAL HSALSDRDEA VNKALELSLQ
421LNRVSLERDS LSRELLRAIR QKVALTQELE AWQDDMQVVI GQQLRSQRQK ELSASASSST
481PRRAAPRFSL RLGPGPAGGF LSNLFRRT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

508 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.