LSBio
CCDC64 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC64 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC64 antibodies →
$725.00each
Catalog Number: LS-G102871-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC64 (NM_207311), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_207311

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAFCLGLVG RASAPAEPDS ACCMELPAAA GDAVRSPAAA AALIFPGGSG ELELALEEEL
61ALLAAGERPS DPGEHPQAEP GSLAEGAGPQ PPPSQDPELL SVIRQKEKDL VLAARLGKAL
121LERNQDMSRQ YEQMHKELTD KLEHLEQEKH ELRRRFENRE GEWEGRVSEL ESDVKQLQDE
181LERQQIHLRE ADREKSRAVQ ELSEQNQRLL DQLSRASEVE RQLSMQVHAL REDFREKNSS
241TNQHIIRLES LQAEIKMLSD RKRELEHRLS ATLEENDLLQ GTVEELQDRV LILERQGHDK
301DLQLHQSQLE LQEVRLSCRQ LQVKVEELTE ERSLQSSAAT STSLLSEIEQ SMEAEELEQE
361REQLRLQLWE AYCQVRYLCS HLRGNDSADS AVSTDSSMDE SSETSSAKDV PAGSLRTALN
421ELKRLIQSIV DGMEPTVTLL SVEMTALKEE RDRLRVTSED KEPKEQLQKA IRDRDEAIAK
481KNAVELELAK CRMDMMSLNS QLLDAIQQKL NLSQQLEAWQ DDMHRVIDRQ LMDTHLKERS
541QPAAALCRGH SAGRGDEPSI AEGKRLFSFF RKI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

573 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.