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CCDC63 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC63 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC63 antibodies →
$504.00each
Catalog Number: LS-G106125-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC63 (NM_152591), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC63
Origin Species
Human
NCBI GenBank
AI123295
SwissProt
Q8NA47

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSVLKKNRRK DSDTPQEPSE KAKEQQAEAE LRKLRQQFRK MVESRKSFKF RNQQKIASQY
61KEIKTLKTEQ DEITLLLSLM KSSRNMNRSE KNYMELRLLL QTKEDYEALI KSLKVLLAEL
121DEKILQMEKK IANQKQIFAK MQEANNPRKL QKQIHILETR LNLVTVHFDK MLTTNAKLRK
181EIEDLRFEKA AYDNVYQQLQ HCLLMEKKTM NLAIEQSSQA YEQRVEAMAR MAAMKDRQKK
241DTSQYNLEIR ELERLYAHES KLKSFLLVKL NDRNEFEEQA KREEALKAKK HVKKNRGESF
301ESYEVAHLRL LKLAESGNLN QLIEDFLAKE EKNFARFTYV TELNNDMEMM HKRTQRIQDE
361IILLRSQQKL SHDDNHSVLR QLEDKLRKTT EEADMYESKY GEVSKTLDLL KNSVEKLFKK
421INCDATKILV QLGETGKVTD INLPQYFAII EKKTNDLLLL ETYRRILEVE GAEAEIPPPF
481INPFWGGSAL LKPPEPIKVI PPVLGADPFS DRLDDVEQPL DHSSLRQLVL DNYILKENRS
541KEVRGDSLPE KVDDFRSRKK VTM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

563 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.