LSBio
CCDC61 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC61 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC61 antibodies →
$725.00each
Catalog Number: LS-G120190-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC61 (NM_001080402), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001267723

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRMHTRLSLP GPPHHVGAAY PPAGGNRSEN AGSWGGAGAS SVEPLARRVA SGEATLAMDQ
61PAGLQVDYVF RGVEHAVRVM VSGQVLELEV EDRMTADQWR GEFDAGFIED LTHKTGNFKQ
121FNIFCHMLES ALTQSSESVT LDLLTYTDLE SLRNRKMGGR PGSLAPRSAQ LNSKRYLILI
181YSVEFDRIHY PLPLPYQGKP DPVVLQGIIR SLKEELGRLQ GLDGQNTRDT RENEIWHLRE
241QVSRLASEKR ELEAQLGRSR EEALAGRAAR QEAEALRGLV RGLELELRQE RGLGHRVAGR
301RGQDCRRLAK ELEEAKASER SLRARLKTLT SELALYKRGG RGSRGRGRPA RPSPSPTGGR
361ALRFDPTAFV KAKERKQREI QMKQQQRNRL GSGGSGDGPS VSWSRQTQPP AALTGRGDAP
421NRSRNRSSSV DSFRSRCSSA SSCSDLEDFS ESLSRGGHRR RGKPPSPTPW SGSNMKSPPV
481ERSHHQKSLA NSGGWVPIKE YSSEHQAADM AEIDARLKAL QEYMNRLDMR S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

531 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.