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CCDC60 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC60 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC60 antibodies →
$504.00each
Catalog Number: LS-G104640-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC60 (NM_178499), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC60
Origin Species
Human
SwissProt
Q8IWA6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTKVPATKKL QSSPNSGAVR PFYASENLRQ VPDKPMKSIK YMDKEIINLK KDLIRSRFLI
61QSVKIGRGYF AILREETAKK KKQQQLQKLK EEERNKFQPA EKISEIHYGD TLLSTYDDEK
121LKTLGARVTR RPFTPIHSCI ISPSLTEAHV EPLFRQLCAL HWLLEALTID HTHHTMKPVI
181TCWNPKDPGG SKSTIKKINK DKSMGQKWEH FITAPKTKEF KIPTMRVTNR KPSRRGSTLS
241LSRASGGSSP QSSMISVNPG SDEPPSVNTQ VTSSKDIEDN ESSSTKPDEE PLYMNLQKLL
301EMVREDARRT VTIENGMQRK APSILSVLKQ NKSNSAYKEM QTTLKSSERS SSTSAESHIQ
361PVQKKSKNRT NCDINIHYKS GVCNTMRAKF YSVAQEAGFC LQDKMEILMK RQEERGIQKF
421RAFVLVSNFQ KDIAKMRHHI SVVKGDAEEI ADHWYFDLLS KLPEDLKNFR PAKKILVKLQ
481KFGENLDLRI RPHVLLKVLQ DLRIWELCSP DIAVAIEFVR EHIIHMPQED YISWLQSRIN
541IPIGPYSALR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

550 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.