LSBio
CCDC55 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC55 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NSRP1 antibodies →
$725.00each
Catalog Number: LS-G109028-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NSRP1 (NM_032141), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP434K1421 | NSrp70 | NSRP1 | CCDC55 | HSPC095
Origin Species
Human
NCBI GenBank
NM_032141
SwissProt
Q9H0G5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MAIPGRQYGL ILPKKTQQLH PVLQKPSVFG NDSDDDDETS VSESLQREAA KKQAMKQTKL
61EIQKALAEDA TVYEYDSIYD EMQKKKEENN PKLLLGKDRK PKYIHNLLKA VEIRKKEQEK
121RMEKKIQRER EMEKGEFDDK EAFVTSAYKK KLQERAEEEE REKRAAALEA CLDVTKQKDL
181SGFYRHLLNQ AVGEEEVPKC SFREARSGIK EEKSRGFSNE VSSKNRIPQE KCILQTDVKV
241EENPDADSDF DAKSSADDEI EETRVNCRRE KVIETPENDF KHHRSQNHSR SPSEERGHST
301RHHTKGSRTS RGHEKREDQH QQKQSRDQEN HYTDRDYRKE RDSHRHREAS HRDSHWKRHE
361QEDKPRARDQ RERSDRVWKR EKDREKYSQR EQERDRQQND QNRPSEKGEK EEKSKAKEEH
421MKVRKERYEN NDKYRDREKR EVGVQSSERN QDRKESSPNS RAKDKFLDQE RSNKMRNMAK
481DKERNQEKPS NSESSLGAKH RLTEEGQEKG KEQERPPEAV SKFAKRNNEE TVMSARDRYL
541ARQMARVNAK TYIEKEDD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

558 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.