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CCDC39 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC39 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC39 antibodies →
$725.00each
Catalog Number: LS-G127558-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC39 (NM_181426), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC39 | CILD14 | FAP59
Origin Species
Human
NCBI GenBank
NM_181426
SwissProt
Q9UFE4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MSSEFLAELH WEDGFAIPVA NEENKLLEDQ LSKLKDERAS LQDELREYEE RINSMTSHFK
61NVKQELSITQ SLCKARERET ESEEHFKAIA QRELGRVKDE IQRLENEMAS ILEKKSDKEN
121GIFKATQKLD GLKCQMNWDQ QALEAWLEES AHKDSDALTL QKYAQQDDNK IRALTLQLER
181LTLECNQKRK ILDNELTETI SAQLELDKAA QDFRKIHNER QELIKQWENT IEQMQKRDGD
241IDNCALELAR IKQETREKEN LVKEKIKFLE SEIGNNTEFE KRISVADRKL LKCRTAYQDH
301ETSRIQLKGE LDSLKATVNR TSSDLEALRK NISKIKKDIH EETARLQKTK NHNEIIQTKL
361KEITEKTMSV EEKATNLEDM LKEEEKDVKE VDVQLNLIKG VLFKKAQELQ TETMKEKAVL
421SEIEGTRSSL KHLNHQLQKL DFETLKQQEI MYSQDFHIQQ VERRMSRLKG EINSEEKQAL
481EAKIVELRKS LEEKKSTCGL LETQIKKLHN DLYFIKKAHS KNSDEKQSLM TKINELNLFI
541DRSEKELDKA KGFKQDLMIE DNLLKLEVKR TREMLHSKAE EVLSLEKRKQ QLYTAMEERT
601EEIKVHKTML ASQIRYVDQE RENISTEFRE RLSKIEKLKN RYEILTVVML PPEGEEEKTQ
661AYYVIKAAQE KEELQREGDC LDAKINKAEK EIYALENTLQ VLNSCNNNYK QSFKKVTPSS
721DEYELKIQLE EQKRAVDEKY RYKQRQIREL QEDIQSMENT LDVIEHLANN VKEKLSEKQA
781YSFQLSKETE EQKPKLERVT KQCAKLTKEI RLLKDTKDET MEEQDIKLRE MKQFHKVIDE
841MLVDIIEENT EIRIILQTYF QQSGLELPTA STKGSRQSSR SPSHTSLSAR SSRSTSTSTS
901QSSIKVLELK FPASSSLVGS PSRPSSASSS SSNVKSKKSS K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

941 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.