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CCDC37 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC37 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC37 antibodies →
$725.00each
Catalog Number: LS-G104168-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC37(CFAP100) (NM_182628), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC37
Origin Species
Human
SwissProt
Q494V2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSEIPSTIVS KNMTNDKNSL ESMNISSSSS TEENPKKQAR KNEEHGPDPS ANPFHLSGDV
61DFFLLRDQER NKALSERQQQ KTMRVHQKMT YSSKVSAKHT SLRRQLQLED KQEDLEARAE
121AEHQRAFRDY TTWKLTLTKE KNVEPENMSG YIKQKRQMFL LQYALDVKRR EIQRLETLAT
181KEEARLERAE KSLEKDAALF DEFVRENDCS SVQAMRAAEK ETKAKIEKIL EIRDLTTQIV
241NIKSEISRFE DTLKHYKVYK DFLYKLSPKE WLEEQEKKHS FLKKAKEVSE ASKESSVNST
301PGDKGPGIKG KASSMWAKEG QGTKKPWRFL QTMRLGRSPS YLSSPQQGSQ PSESSGGDSR
361GSNSPIPPTQ EDTDSDGEEP QLYFTEPQQL LDVFRELEEQ NLSLIQNSQE TEKTLEELSH
421TLKHTQIRMD REVNQLKQWV TTMMMSITKE EDTAAELELK ARVFHFGEYK GDQQDKLLES
481LNCKVLDVYR HCTGTQQEAN LGTVQMLTII EHQLDELLEN LEHVPQVKIE QAERAKEKER
541RIRLREEKLQ MQKILQEEHL QRARARAQAE IKKKRGRTLV CRSRPPAHRI KQQSEHTLMD
601KEEEELLFFF T
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

611 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.