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CCDC36 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC36 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC36 antibodies →
$504.00each
Catalog Number: LS-G104757-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC36 (NM_178173), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC36 | CT74 | Cancer/testis antigen 74
Origin Species
Human
NCBI GenBank
NM_001135197
SwissProt
Q8IYA8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLSIPSGSGN KKSSNWNNNQ NDYSSLSDSQ FLFGSQFCPE NSETLSAPLD FGAHLRHSKQ
61SQQNYLEGEP SIFTKYQTKP QLFGGDIKDG GLFPPPLSVG KSKGLLEQFE EKKKRAKDKC
121DSETLYNFVS NVRESILRLQ TSVEKSEDHL SSRSQSILDS LETVAKTLQE TIQAQNDLVF
181EAVQDKGNME QAILEMKKRF EARQGEFIEM KSNLKHLEVL VAQQSQEFQQ LCEQLGQLNV
241PSVLAELKRL ISVPPVKDSA SQTSPPLAQS LNLTRQEKYT SEKPVLWQAQ ALPAAWNPGM
301GSLQPGEFDV WGEGAKNDDL QEEAALPAFG SHERNRHVKD KVVQTNCKNW AVTKTGAKNH
361GSSVPGHKIP SDRDLVSQGA SQLTSLEINF STSIKNACQK YQAQSMFLCD PREHLVIKQK
421DGTVEMRGKD KKQQPRKAHR AHRGRLIASK QKQIPIQTCK FNSKYQSPQP AISVPQSPFL
481GQQEPRAQPL HLQCPRSPRK PVCPILGGTV MPNKTVRAVQ GRLLQLSRCS SQDNWLLSSS
541SQGDHQMSWF SDLNLGCSET PLCKEAGKNL LYDLGFDSSD DDGF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

584 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.