Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCDC186 / C10orf118 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112057-100
Catalog Number: LS-G112057
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C10orf118(CCDC186) (NM_018017), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C10orf118 | CTCL tumor antigen L14-2
- Origin Species
- Human
- NCBI GenBank
- NM_018017
- SwissProt
- Q7Z3E2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 103.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSETDHIAST SSDKNVGKTP ELKEDSCNLF SGNESSKLEN ESKLLSLNTD KTLCQPNEHN61NRIEAQENYI PDHGGGEDSC AKTDTGSENS EQIANFPSGN FAKHISKTNE TEQKVTQILV121ELRSSTFPES ANEKTYSESP YDTDCTKKFI SKIKSVSASE DLLEEIESEL LSTEFAEHRV181PNGMNKGEHA LVLFEKCVQD KYLQQEHIIK KLIKENKKHQ ELFVDICSEK DNLREELKKR241TETEKQHMNT IKQLESRIEE LNKEVKASRD QLIAQDVTAK NAVQQLHKEM AQRMEQANKK301CEEARQEKEA MVMKYVRGEK ESLDLRKEKE TLEKKLRDAN KELEKNTNKI KQLSQEKGRL361HQLYETKEGE TTRLIREIDK LKEDINSHVI KVKWAQNKLK AEMDSHKETK DKLKETTTKL421TQAKEEADQI RKNCQDMIKT YQESEEIKSN ELDAKLRVTK GELEKQMQEK SDQLEMHHAK481IKELEDLKRT FKEGMDELRT LRTKVKCLED ERLRTEDELS KYKEIINRQK AEIQNLLDKV541KTADQLQEQL QRGKQEIENL KEEVESLNSL INDLQKDIEG SRKRESELLL FTERLTSKNA601QLQSESNSLQ SQFDKVSCSE SQLQSQCEQM KQTNINLESR LLKEEELRKE EVQTLQAELA661CRQTEVKALS TQVEELKDEL VTQRRKHASS IKDLTKQLQQ ARRKLDQVES GSYDKEVSSM721GSRSSSSGSL NARSSAEDRS PENTGSSVAV DNFPQVDKAM LIERIVRLQK AHARKNEKIE781FMEDHIKQLV EEIRKKTKII QSYILREESG TLSSEASDFN KVHLSRRGGI MASLYTSHPA841DNGLTLELSL EINRKLQAVL EDTLLKNITL KENLQTLGTE IERLIKHQHE LEQRTKKTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
898 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.