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CCDC17 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC17 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC17 antibodies →
$504.00each
Catalog Number: LS-G131056-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC17 (NM_001190182), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC17 | RP4-697E16.4
Origin Species
Human
NCBI GenBank
NM_001114938

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67 kda
Protein Type
Lysate
Amino Acid Sequence
1MDSHSGEPAL LPCGTCDMVF RSSALLATHT QRFCIGHPTQ EMTFGAQASV ATEPQRAAVV
61PQEHQGVPQE PQGLPDQQAS RSALKRLTEE EMRPWITEVP RVFAGPWTRS EARPQSPMSE
121AVGSPSERLR ALFRTRARRV AEMEAQSRAL QLRGEELSRR LQVVACTRGG MSRLFGLEQE
181IRELQAEAGR TRGALEVLGA RIQELQAEPG NPLSSRREAE LYSPVQKANP GTLAAEIRAL
241REAYIRDGGR DPGVLGQIWQ LQVEASALEL QRSQTRRGRA GATSGELPVV EAENRRLEAE
301ILALQMQRGR APLGPQDLRL LGDASLQPKG RRDPPLLPPP VAPPLPPLPG FSEPQLPGTM
361TRNLGLDSHF LLPTSDMLGP APYDPGAGLV IFYDFLRGLE ASWIWVQLRT GLARDGRDTG
421RTTALPPALC LPPPPAPGPM GNCAILASRQ PVPRLPPSSS VSLVCELQVW QGLAWARAPQ
481PKAWVSLGLF DQDQRVLSGR WRLPLRALPL DPSLSLGQLN GIPQAGQAEL FLRLVNARDA
541AVQTLAEINP ASVHEYQYPP PVSSTSSLEA SFLTPAVGFA DPPPRTEEPL SGVKDRDEGL
601GPHHSSDLPP VSF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

613 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.