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CCDC157 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC157 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC157 antibodies →
$504.00each
Catalog Number: LS-G121270-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC157 (NM_001017437), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1656 | CCDC157
Origin Species
Human
SwissProt
Q569K6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVALLEHYDH VPGDPEFTQL SHAVLLELVI DRLLLLLQSC MSYLENLGSE QMMPPAQAAG
61PCMSVGLTVR RFWDSLLRLG TLHQQPLPQK GANQRETPTS KPTTKGEPAR SPEYLTTKLI
121KPSSPVLGLP QTCQEPESIP VRASLQFPAT TFKNTRSVHS QTIETALVPC DACASVQGSL
181QKVGKVVISL CQSQNLPSSL GQFQQLVQDS MGLRPLPAAT VGRWAAEQRK DLTRLSKHVE
241ALRAQLEEAE GQKDGLRKQA GKLEQALKQE QGARRRQAEE DEQCLSEWEH DKQQLLTETS
301DLKTKMATLE RELKQQREST QAVEAKAQQL QEEGERRAAA ERQVQQLEEQ VQQLEAQVQL
361LVGRLEGAGQ QVCWASTELD KEKARVDSMV RHQESLQAKQ RALLKQLDSL DQEREELRGS
421LDEAEAQRAR VEEQLQSERE QGQCQLRAQQ ELLQSLQREK QGLEQATTDL RLTILELERE
481LEELKERERL LVAFPDLHRP TETQIHGGRS SSVESQITCP TDSGNVTDHM ERQVQANDIR
541IRVLQEENGR LQSMLSKIRE VAQQGGLKLI PQDRLWSPSS KGTQGATPPV QAKSTSPGPL
601GRQHLPSSRT GRTLLGQPCT SPPRQPCTSP PRQPCTSPPR QPCTSPSRQP CSQPSKSLLE
661GVTHLDTCTQ NPIKVLVRLR KRLSPGRGQA SSAHQPQERP M
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

701 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.