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CCDC155 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC155 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC155 antibodies →
$504.00each
Catalog Number: LS-G106894-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC155 (NM_144688), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_144688

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLPEGPVGG PTAEMYLRER PEEARLGMPV SLEEQILNST FEACDPQRTG TVAVAQVLAY
61LEAVTGQGPQ DARLQTLANS LDPNGEGPKA TVDLDTFLVV MRDWIAACQL HGGLELEEET
121AFQGALTSQQ LPSGCPEAEE PANLESFGGE DPRPELQATA DLLSSLEDLE LSNRRLVGEN
181AKLQRSMETA EEGSARLGEE ILALRKQLHS TQQALQFAKA MDEELEDLKT LARSLEEQNR
241SLLAQARQAE KEQQHLVAEM ETLQEENGKL LAERDGVKKR SQELAMEKDT LKRQLFECEH
301LICQRDTILS ERTRDVESLA QTLEEYRVTT QELRLEISRL EEQLSQTYEG PDELPEGAQL
361RRVGWTELLP PSLGLEIEAI RQKQEVATAD LSNPLCGVWQ WEEVIHETSE ETEFPSEAPA
421GGQRNFQGEP AHPEEGRKEP SMWLTRREEE EDAESQVTAD LPVPLGAPRP GDIPENPPER
481PARRELQQAL VPVMKKLVPV RRRAWGQLCL PPQRLRVTRH PLIPAPVLGL LLLLLLSVLL
541LGPSPPPTWP HLQLCYLQPP PV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

562 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.