LSBio
CCDC154 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC154 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC154 antibodies →
$725.00each
Catalog Number: LS-G126275-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC154 (NM_001143980), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001143980

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSELADSGPS GASAPSQLRA VTLEDLGLLL AGGLASPEPL SLEELSERYE SSHPTSTASV
61PEQDTAKHWN QLEQWVVELQ AEVACLREHK QRCERATRSL LRELLQVRAR VQLQGSELRQ
121LQQEARPAAQ APEKEAPEFS GLQNQMQALD KRLVEVREAL TRLRRRQVQQ EAERRGAEQE
181AGLRLAKLTD LLQQEEQGRE VACGALQKNQ EDSSRRVDLE VARMQAQVTK LGEEVSLRFL
241KREAKLCGFL QKSFLALEKR MKASESSRLK LEGSLRGELE SRWEKLRGLM EERLRALQGQ
301HEESHLLEQC QGLDAAVAQL TKFVQQNQAS LNRVLLAEEK AWDAKGRLEE SRAGELAAYV
361QENLEAAQLA GELARQEMHG ELVLLREKSR ALEASVAQLA GQLKELSGHL PALSSRLDLQ
421EQMLGLRLSE AKTEWEGAER KSLEDLARWR KEVTEHLRGV REKVDGLPQQ IESVSDKCLL
481HKSDSDLRIS AEGKAREFKV GALRQELATL LSSVQLLKED NPGRKIAEMQ GKLATFQNQI
541MKLENCVQAN KTIQNLRFNT EARLRTQEMA TLWESVLRLW SEEGPRTPLG SWKALPSLVR
601PRVFIKDMAP GKVVPMNCWG VYQAVRWLRW KASLIKLRAL RRPGGVLEKP HSQEQVQQLT
661PSLFIQK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

667 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.