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CCDC151 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC151 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC151 antibodies →
$504.00each
Catalog Number: LS-G106777-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC151 (NM_145045), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC151
Origin Species
Human
NCBI GenBank
AA843771
SwissProt
A5D8V7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTSPLCRAAS ANALPPQDQA STPSSRVKGR EASGKPSHLR GKGTAQAWTP GRSKGGSFHR
61GAGKPSVHSQ VAELHKKIQL LEGDRKAFFE SSQWNIKKNQ ETISQLRKET KALELKLLDL
121LKGDEKVVQA VIREWKWEKP YLKNRTGQAL EHLDHRLREK VKQQNALRHQ VVLRQRRLEE
181LQLQHSLRLL EMAEAQNRHT EVAKTMRNLE NRLEKAQMKA QEAEHITSVY LQLKAYLMDE
241SLNLENRLDS MEAEVVRTKH ELEALHVVNQ EALNARDIAK NQLQYLEETL VRERKKRERY
301ISECKKRAEE KKLENERMER KTHREHLLLQ SDDTIQDSLH AKEEELRQRW SMYQMEVIFG
361KVKDATGTDE THSLVRRFLA QGDTFAQLET LKSENEQTLV RLKQEKQQLQ RELEDLKYSG
421EATLVSQQKL QAEAQERLKK EERRHAEAKD QLERALRAMQ VAKDSLEHLS SKLIHITVED
481GRFAGKELDP QADNYVPNLL GLVEEKLLKL QAQLQGHDVQ EMLCHIANRE FLASLEGRLP
541EYNTRIALPL ATSKDKFFDE ESEEEDNEVV TRASLKIRSQ KLIESHKKHR RSRRS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

595 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.