LSBio
CCDC146 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC146 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC146 antibodies →
$504.00each
Catalog Number: LS-G110929-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC146 (NM_020879), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC146 | KIAA1505
Origin Species
Human
SwissProt
Q8IYE0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
112.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEDSSTDTEK EEEEEKDEKD QEPIYAIVPT INIQDERFVD LSETPAFIFL HELHAMGKLP
61GTRMAALKAK YTLLHDAVMS TQESEVQLLQ NAKRFTEQIQ QQQFHLQQAD NFPEAFSTEV
121SKMREQLLKY QNEYNAVKER EFHNQYRLNS LKEEKIIIVK EFEKITKPGE MEKKMKILRE
181STEELRKEIM QKKLEIKNLR EDLASKQKQL LKEQKELEEL LGHQVVLKDE VAHHQTIPVQ
241IGKEIEKITR KKVEMEKKKI VLQQEVKTLN DSLKKVENKV SAIVDEKENV IKEVEGKRAL
301LEIKEREHNQ LVKLLELARE NEATSLTERG ILDLNLRNSL IDKQNYHDEL SRKQREKERD
361FRNLRKMELL LKVSWDALRQ TQALHQRLLL EMEAIPKDDS TLSERRRELH KEVEVAKRNL
421AQQKIISEME SKLVEQQLAE ENKLLKEQEN MKELVVNLLR MTQIKIDEKE QKSKDFLKAQ
481QKYTNIVKEM KAKDLEIRIH KKKKCEIYRR LREFAKLYDT IRNERNKFVN LLHKAHQKVN
541EIKERHKMSL NELEILRNSA VSQERKLQNS MLKHANNVTI RESMQNDVRK IVSKLQEMKE
601KKEAQLNNID RLANTITMIE EEMVQLRKRY EKAVQHRNES GVQLIEREEE ICIFYEKINI
661QEKMKLNGEI EIHLLEEKIQ FLKMKIAEKQ RQICVTQKLL PAKRSLDADL AVLQIQFSQC
721TDRIKDLEKQ FVKPDGENRA RFLPGKDLTE KEMIQKLDKL ELQLAKKEEK LLEKDFIYEQ
781VSRLTDRLCS KTQGCKQDTL LLAKKMNGYQ RRIKNATEKM MALVAELSMK QALTIELQKE
841VREKEDFIFT CNSRIEKGLP LNKEIEKEWL KVLRDEEMHA LAIAEKSQEF LEADNRQLPN
901GVYTTAEQRP NAYIPEADAT LPLPKPYGAL APFKPSEPGA NMRHIRKPVI KPVEI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

955 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.