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CCDC144B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC144B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC144B antibodies →
$504.00each
Catalog Number: LS-G104253-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC144B (NM_182568), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
SwissProt
Q3MJ40

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASWGGEKRG GAEGSPKLAV YATRKTRSVR SQEDQWYLGY PGDQWSSGFS YSWWKNSVGS
61ESKHGEGALD QPQHDVRLED LGELHRAARS GDVPGVEHVL APGDTGVDKR DRKKSIQQLV
121PEYKEKQTPE SLPQNNNPDW HPTNLTLSDE TCQRSKNLKV GDKSPSVSPS MPENQSATKE
181LGQMNLTERE KMDTGVVLLS GNDTLHDLCQ SQLPENKESK EAEQDLELTS EEEQERLKGC
241ENKQPQKTSQ EPEMAKDCDR EDIPIYPVLP HVQKSEEMWI EQGKLEWKNQ LKLVINELKQ
301RFGEIYEKYK IPACPEEEPL LDNSTRGTDV KDIPFNLTNN IPGCEEEDAS EISVSVVFET
361FPEQKEPSLK NIIHPYYHPY SGSQEHVCQS SSKLHLHENK LDCDNDNKLG IGHIFSTDNN
421FHNDASTKKA RNPEVVTVEM KEDQEFDLQM TKNMNQNSDS GSTNNYKSLK PKLENLSSLP
481PDSDRTSEVY LHEELQQDMQ KFKNEVNTLE EEFLALKKEN VQLHKEVEEE MEKHRSNSTE
541LSGTLTDGTT VGNDDDGLNQ QIPRKENEEH DRPADKTANE KNKVKNQIYP EADFADSMEP
601SEIASEDCEL SHSVYENFML LIEQLRMEYK DSASLPRIQD TFCLCEHLLK LKNNHCDQLT
661VKLKQMENMV SVLQNELSET KKTKLQLELQ KIEWEKELYD LRLALKQENG RKEMPICCII
721KIVNS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

725 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.