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CCDC142 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC142 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC142 antibodies →
$725.00each
Catalog Number: LS-G108621-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC142 (NM_032779), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC142 | FLJ14397
Origin Species
Human
NCBI GenBank
NM_032779
SwissProt
Q17RM4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAQASRSGSL PPLVIVPPLR AQPGGTGEEQ WERSRTGGLR WEVHCWPSGT SGGTPWWPTP
61ADVSEDYEAD AAAWRRGPAG GGPIPPALQR LRAVLLRLHR EREQLLQARD CAYHLQSAVR
121LMKTLSPGSP SGGPSPLPQW CRDLQLHPSQ GAVLRIGPGE TLEPLLLARP IGLAAQCLEA
181VIEMQLRALG REPASPGLSS QLAELLFALP AYHTLQRKAL SHVPGAARPF PTSRVLRLLT
241GERGCQVASR LDEALQGSAL RDQLRRRCQE EGDLLPGLLG LVGGVAGSAS CGLGLGGAGA
301LWSQYWTLLW AACAQSLDLN LGPWRDPRAT AQQLSQALGQ ASLPQECEKE LASLCHRLLH
361QSLIWSWDQG FCQALGSALG GQSSLPTSSG TAELLQQLFP PLLDALREPR LRRIFCQPAG
421LCTLQTTLLW FLGRAQQYLA AWDPASFLLL IQKDLPPLLH EAEALYSLAS EESLALEVEQ
481QLGLEIQKLT AQIQLLPEES LSVFSQECHK QAMQGFKLYM PRGRYWRLRL CPEPPSAPSE
541YAGLVVRTVL EPVLQGLQGL PPQAQAPALG QALTAIVGAW LDHILTHGIR FSLQGALQLK
601QDFGVVRELL EEEQWSLSPD LRQTLLMLSI FQQLDGALLC LLQQPLPKSQ VHRRPPCCCA
661CQEVQTTKLP SSCLNSLESL EPPLQPGTSP AQTGQLQSTL GGRGPSPEGY LVGNQQAWLA
721LRQHQRPRWH LPFFSCLGTS PES
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

743 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.