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CCDC141 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC141 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC141 antibodies →
$725.00each
Catalog Number: LS-G105265-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC141 (NM_173648), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC141 | CAMDI
Origin Species
Human
SwissProt
Q6ZP82

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQFQSLKEFY ETEIPQKEQD DAKAKHCSDS AEKQWQLFLK KSFITQDLGL EFLNLINMAK
61ENEILDVKNE VYLMKNTMEN QKAEREELSL LRLAWQLKAT ESKPGKQQWA AFKEQLKKTS
121HNLKLLQEAL MPVSALDLGG SLQFILDLRQ KWNDMKPQFQ QLNDEVQYIM KESEELTGRG
181APVKEKSQQL KDLIHFHQKQ KERIQDYEDI LYKVVQFHQV KEELGRLIKS RELEFVEQPK
241ELGDAHDVQI HLRCSQEKQA RVDHLHRLAL SLGVDIISSV QRPHCSNVSA KNLQQQLELL
301EEDSMKWRAK AEEYGRTLSR SVEYCAMRDE INELKDSFKD IKKKFNNLKF NYTKKNEKSR
361NLKALKYQIQ QVDMYAEKMQ ALKRKMEKVS NKTSDSFLNY PSDKVNVLLE VMKDLQKHVD
421DFDKVVTDYK KNLDLTEHFQ EVIEECHFWY EDASATVVRV GKYSTECKTK EAVKILHQQF
481NKFIAPSVPQ QEERIQEATD LAQHLYGLEE GQKYIEKIVT KHKEVLESVT ELCESLTELE
541EKLKQGDVLK MNPNLEDFHY DYIDLLKEPA KNKQTIFNEE RNKGQVQVAD LLGINGTGEE
601RLPQDLKVST DKEGGVQDLL LPEDMLSGEE YECVSPDDIS LPPLPGSPES PLAPSDMEVE
661EPVSSSLSLH ISSYGVQAGT SSPGDAQESV LPPPVAFADA CNDKRETFSS HFERPYLQFK
721AEPPLTSRGF VEKSTALHRI SAEHPESMMS EVHERALQQH PQAQGGLLET REKMHADNNF
781TKTQDRLHAS SDAFSGLRFQ SGTSRGYQRQ MVPREEIKST SAKSSVVSLA DQAPNFSRLL
841SNVTVMEGSP VTLEVEVTGF PEPTLTWWVA YNDKP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

875 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.