LSBio
CCDC136 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC136 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC136 antibodies →
$725.00each
Catalog Number: LS-G110261-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC136 (NM_022742), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_022742

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
133.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQAMEGEVLL PALYEEEEEE EEEEEEVEEE EEQVQKGGSV GSLSVNKHRG LSLTETELEE
61LRAQVLQLVA ELEETRELAG QHEDDSLELQ GLLEDERLAS AQQAEVFTKQ IQQLQGELRS
121LREEISLLEH EKESELKEIE QELHLAQAEI QSLRQAAEDS ATEHESDIAS LQEDLCRMQN
181ELEDMERIRG DYEMEIASLR AEMEMKSSEP SGSLGLSDYS GLQEELQELR ERYHFLNEEY
241RALQESNSSL TGQLADLESE RTQRATERWL QSQTLSMTSA ESQTSEMDFL EPDPEMQLLR
301QQLRDAEEQM HGMKNKCQEL CCELEELQHH RQVSEEEQRR LQRELKCAQN EVLRFQTSHS
361VTQNEELKSR LCTLQKKYDT SQDEQNELLK MQLQLQTELR QLKVMKSTLV ENQSEKELLC
421RLQKLHLQHQ NVTCEKEKLL ERQQQLQEEL QCHEAELQHL RDTVASFKES NEKDTETHAQ
481LQEMKQLYQA SKDELERQKH MYDQLEQDLL LCQLELKELK ASHPIPEDKG KCANKCDTLL
541SRLTELQEKY KASQKEMGQL QMEQCELLED QRRMQEEQGQ LQEELHRLTL PLPKSGLLLK
601SQELLTKLED LCELQLLYQG MQEEQKKLIQ NQDCVLKEQL EIHEELRRFK ESHFQEVLEN
661PDDSKLAKSS KCNRNKQSKL LMEQMQALQV MYDAGQAKQE LLQQEQGRLL EERKRLQADL
721QLCLEEMQLL QVQSPSIKMS LESYGKSYGS MVPSNENCRK TYDTTVDDNE SYYKSYTSTQ
781TSSKSFLKSY DSSTSASEAY GKSYCTTSNS SITYKKSYGS TSSSDTCQKS FVSSCTDEEP
841AEPEDMERFE EMVVKVLIKL QAVQAMYQIS QEEHSQLQEQ MEKLLAKQKD LKEELDACER
901EFKECMECLE KPMAPQNDKN EIKELQTKLR ELQLQYQASM DEQGRLLVVQ EQLEGQLQCC
961QEELRQLREK RPSVVKEARG KNANKNMNKN ANGVKMKKVT KPCSDTSESD LETRKSLEVV
1021LYYKASQRKL DGLAKEEEKK EEMEEEKKQV KEEAKEQCGD ELVAEPADPE EAKSTEDQEE
1081NEEDKEEEEK EEDSEEEEDD ADSSLESPEE NNPLRLSESK KSSPTPNPPI FSLPLVGLVV
1141ISALLWCWWA ETSS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1154 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.