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CCDC13 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC13 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC13 antibodies →
$504.00each
Catalog Number: LS-G106883-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC13 (NM_144719), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC13
Origin Species
Human
NCBI GenBank
NM_144719
SwissProt
Q8IYE1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAADESSQNT LRLQFKAMQE MQHKRLQKQM EKKREKELSL KSRADDQEEP LEVSDGLSLL
61HAGEPNSKNS FEKRVLEDEI EHLRNELRET VDENGRLYKL LKERDFEIKH LKKKIEEDRF
121AFTGTAGVAG DVVATKIVEL SKKNRLLMAE SEGAKTRVKQ LTNRIQELER ELQTALTRLS
181AKGATDAGAK PPRAQMGDRA LLETPEVKAL QDRLVATNLK MSDLRNQIQS VKQELRMAQK
241VLAREVGEDI NVQQLLSSPG TWRGRAQQIL VLQSKVQELE KQLGQARSQS AGTASDELSV
301YPDPRKLSAQ EKNLLRIRSL EREKQEGLEK LASERDVLQR ELEELKKKFE GMRSRNKLLS
361SEMKTLKSQM GTLVVKGRHD DELIDALMDQ LKQLQEILGS LSLQEEKTRV SQHHLDQQLN
421SEAQRSNSLV AQLQAMVAER EAKVRQLEME IGQLNVHYLR NKGVGEGSSG REVSPAYTQF
481LEDPGLTKSP ASAGDHVGRL GSSRSVTSLG HTLVESALTR PSLPSPHRTS PRFSDSPEQK
541GWQAQVSEIK ALWQAAEVER DRLTEFVTVL QKRVEESNSK LLESERKLQE ERHRTVVLEQ
601HLEKIRLEPG KASASQRAAP RTKTGLPTSN NRHNPTGSEK KDPSFAQLSD VPVESQMEEL
661TTRLAIQVEE NEMLKAALGS ALRGKEEDFR MYHEILGQVK SVFLQALRQQ KTGKQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

715 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.