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CCDC129 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC129 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC129 antibodies →
$504.00each
Catalog Number: LS-G103924-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC129 (NM_194300), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001257967

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
115.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMAQKSQGSD NLQEGQEKSK REILKCTKSA WAPLDEWLPP DPEEESQSLT IPMLEDSKQE
61SIQQWLDSGF FVSANENFQQ VIDRTVSLYE QGMVQMTVKD YMRSLHQFSE TPILSRGTSF
121NSCYSTASVP QSIPEWLEFW EIDPVEILLD LGFGADEPDI CMQIPARFLG CGSAARGINI
181RVFLEAQKQR MDIENPNLYG RFRQLEILDH VTNAFSSLLS DVSILPNRAE EKAGGESVQR
241TSVSAAKEHR RRMGKLLRRA SKQNIRRDCN PEVSESFKVK DEVFVPFTKP WDCGAELAAT
301SINHKQNHLS LSVEHQSLQA CDDLLPYPPH GLLSKQWPCS SMPAKQAPPS CVSEGSVKGR
361TQKENLFQTN KLKSLSHLAG KGPDSFEMEE VQSFEEETGN PLDMTSGTVG ARVDRANSCQ
421SDSSGFLEEP LEPLPLQMPS LPNSQSPAEN GGRKPRDQSH SLVSSQDCQL ESDGPDSKSR
481ASMSFSSQEA NALEQRASVS VMEEEFLLEA MEGPPELYIP DMACAKTTTR GECPRKDSHL
541WQLLPMPHAE YEVTRPTATS KYDHPLGFMV THVTEMQDSF VRPEGAGKVQ SHHNESQRSP
601GNDHTQDKFL HVDSEAPREE ESSGFCPHTN HSLLVPESSS QCIPKHSEIT PYATDLAQTS
661EKLIPHLHKL PGDPAQVKSR SGTLGQILPG TEAEMENLPL NTGSSRSVMT QMSSSLVSAA
721QRAVALGTGP RGTSLECTVC DPVTATETRL GTKARQLNDA SIQTSALSNK TLTHGPQPLT
781KSVSLDSGFS SICPMGTCHA IPAHCCICCH HHPHCHGERQ SPGPEPSVCR HCLCSLTGHQ
841EAQFMTTLKA LQDTTVRELC SCTVHEMEAM KTICQSFREY LEEIEQHLMG QQALFSRDMS
901EEEREEAEQL QTLREALRQQ VAELEFQLGD RAQQIREGIL LQLEVLTAEP PEHYSNLHQY
961NWIEESNGQT SCSKIHPGMA PRTVFPPDDG QEAPCSGGTQ LAAFTPPTLE NSTRMSPSSS
1021AWAKLGPTPL SNCPVGEKDA DVFL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1044 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.