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CCDC122 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC122 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC122 Proteins & Peptides →
$504.00each
Catalog Number: LS-G106829-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC122 (NM_144974), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC122
Origin Species
Hu
NCBI GenBank
AA761347

Protein

Expression System
HEK293
Fusion Tag
FLAG, Myc
Molecular Weight
32 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDNKERKSQ GFPKEDNQDT SSLADAVEKV AKQQQSQASE IEKNKKVLFN LKNELHELEK
61EIAAISAETK ETERQIYQQD SAIENTKLHC DSLETQIKSL HSENVKLKFD IETAQEDFEE
121HMIKYNAYYA KIKAHKNSLG EVESKWSFMT ELHEKRDFVK KLKTMKEELM QDLQNPGGNR
181ITQVQEDITN LKDKIITVKE SIIEKTCFLE EEKKTHEKLR KEIEVQHKRY DAILKRLHCQ
241VNKLQSNRRQ WQWNIQQLEK TAAELRKCIG MQE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

273 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.