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CCDC116 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC116 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC116 antibodies →
$504.00each
Catalog Number: LS-G106143-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC116 (NM_152612), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARCRHHSGY LADDEASHSM CSARVQLPKK PLVPEMRPAC KPGRVPHPPS TCGSSALQGQ
61RRNKRHPQPF GHFLDFLTES QVLDSLETVV EKATERMAAM KTEAGVPLVE VQDPVEVPSG
121GRRAHARPSL STVHRHRVRP TLCTGHPNNY PSSSSSMSNC HSSLMAGCLG SHSRDSDLGA
181QGSLPPVRDK LLLEKNLKRL LQLEREGKGL SQSCSQRDSL LWDSLGSQTS FQWTQEQPLS
241WFSGLLGSSS GVPEASEPRP GEQEPIFRKR EFNKEIKSLL SQLESLDLPG YCPLREPHRT
301LNFLADHRLF PALQSVVSQA VDKLRGAHCR DGRPLFPTSL EPTSDLPPLG SEPAKPTNGG
361QPYASPRPTV SSPKMLQRKR KDRGGSPSMS SAQVATRFKL KSPCSSSRFT KKKPLPSISS
421KSSMSHFSNR LYEELADFLT QQAASLVIRK YEFEKDLSKQ LGFFSFPITH VLRDLSLGLK
481KVKGSRIHLS SETHRSCLLR KLEESKRARQ ASRLSTSHCS TETPSVQQEP ATHTAQDQAT
541EPCRSLYTNL PASRQLSPLE PKLYMSACTG MGSSPPKSKD MDNEGRDKAE IEDEDEDEFK
601DEDQDEDKDE DGV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

613 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.