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CCDC110 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC110 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC110 antibodies →
$504.00each
Catalog Number: LS-G105998-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC110 (NM_152775), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC110 | Cancer/testis antigen KM-HN-1 | Cancer/testis antigen 52 | KM-HN-1 | KMHN1 | CT52
Origin Species
Human
SwissProt
Q8TBZ0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MSPEKQHREE DEVDSVLLSA SKILNSSEGV KESGCSDTEY GCIAESENQI QPQSALKVLQ
61QQLESFQALR MQTLQNVSMV QSEISEILNK SIIEVENPQF SSEKNLVFGT RIEKDLPTEN
121QEENLSMEKS HHFEDSKTLH SVEEKLSGDS VNSLPQSVNV PSQIHSEDTL TLRTSTDNLS
181SNIIIHPSEN SDILKNYNNF YRFLPTAPPN VMSQADTVIL DKSKITVPFL KHGFCENLDD
241ICHSIKQMKE ELQKSHDGEV ALTNELQTLQ TDPDVHRNGK YDMSPIHQDK MNFIKEENLD
301GNLNEDIKSK RISELEALVK KLLPFRETVS KFHVHFCRKC KKLSKSEMHR GKKNEKNNKE
361IPITGKNITD LKFHSRVPRY TLSFLDQTKH EMKDKERQPF LVKQGSIISE NEKTSKVNSV
421TEQCVAKIQY LQNYLKESVQ IQKKVMELES ENLNLKSKMK PLIFTTQSLI QKVETYEKQL
481KNLVEEKSTI QSKLSKTEEY SKECLKEFKK IISKYNVLQG QNKTLEEKNI QLSLEKQQMM
541EALDQLKSKE HKTQSDMAIV NNENNRMSIE MEAMKTNILL IQDEKEMLEK KTHQLLKEKS
601SLGNELKESQ LEIIQLKEKE RLAKTEQETL LQIIETVKDE KLNLETTLQE STAARQIMER
661EIENIQTYQS TAEENFLQEI KNAKSEASIY KNSLSEIGKE CEMLSKMVME TKTDNQILKE
721ELKKHSQENI KFENSISRLT EDKILLENYV RSIENERDTL EFEMRHLQRE YLSLSDKICN
781QHNDPSKTTY ISRREKFHFD NYTHEDTSSP QSRPLASDLK GYFKVKDRTL KHH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

833 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.