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CCDC105 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC105 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC105 antibodies →
$504.00each
Catalog Number: LS-G105330-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC105 (NM_173482), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC105
Origin Species
Human
SwissProt
Q8IYK2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRVLVPPAER SQDTRVGAPA WREAAQAMAR TAHILTDRCG QEAVTMWQPK DSVLDPNVAH
61HLGRAAYIQP WRFRVEMIKG GGTLEKPPPG EGVTLWKGKM KPPAWYARLP LPLHRKARAL
121QTTEVVHAHA RGARLTAARL GRAQHQINGR VRQLLRQREV TDHRLSEVRK GLLINQQSVK
181LRGYRPKSEK VPDKADSMLT WEKEELKSMK RKMERDMEKS EVLLKTLASC RDTLNFCFKE
241RLQAVDLMNQ PLDKVLEQAR RHSWVNLSRA PTPRTQGQKT PPPDPVGTYN PACALALNEA
301KRLLVESKDT LVEMAKNEVD VREQQLQISD RVCASLAQKA SETLELKERL NMTLGLMRGT
361ILRCTKYNQE LYTTHGLIKG PLSKVHLETA EKLDRPLVRM YQRHVGTQLP EAARLAQGTD
421KLQCHITYLE KNLEELLATH KNLTWGLNCK NIGHEVDGNV VRLRLRQRQP HVCYEQAQRL
481VKDWDPRTPP PRSKSSADP
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

499 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.