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CC2D1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CC2D1B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CC2D1B antibodies →
$725.00each
Catalog Number: LS-G102071-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CC2D1B (NM_032449), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_032449
SwissProt
Q5T0F9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMPGPRPRKG PQARGQGVAA AKQMGLFMEF GPEDMLLGMD EAEDDEDLEA ELLALTGEAQ
61TTGKKPAPKG QAPLPMAHIE KLAADCMRDV EEEEEEEGLE EDAELLTELQ EVLGVDEETE
121PLDGDEVADP GGSEEENGLE DTEPPVQTAV LTASAPAAQA GASQGLHALL EERIHNYREA
181AASAKEAGEA AKARRCERGL KTLESQLASV RRGRKINEDE IPPPVALGKR PLAPQEPANR
241SPETDPPAPP ALESDNPSQP ETSLPGISAQ PVSDLDPDPR ALLSSRQREY KVAALSAKRA
301GELDRARELM RIGKRFGAVL EALEKGQPVD LSAMPPAPED LKPQQASQAP TAPSVIPPAV
361ERVQPVMAPD VPATPVAPTE SQTVLDALQQ RLNKYREAGI QARSGGDERK ARMHERIAKQ
421YQDAIRAHRA GRKVNFAELP VPPGFPPIPG LESTMGVEED AVAATLAAAE KLASAEDSAP
481ADKDEDEPPG HLQGEPPAQA PVAKKPARPT VPSSQRLPEP RASSSKESPS PSVREQLALL
541EARKLQYQRA ALQAKRSQDL EQAKAYLRVA KWLEAQIIQA RSGRPVDLSK VPSPLTDEEG
601DFILIHHEDL RLSQKAEEVY AQLQKMLLEQ QEKCLLFSKQ FMHQGNVAET TRFEKLAQDR
661KKQLEILQLA QAQGLDPPTH HFELKTFQTV RIFSELNSTE MHLIIVRGMN LPAPPGVTPD
721DLDAFVRFEF HYPNSDQAQK SKTAVVKNTN SPEFDQLFKL NINRNHRGFK RVIQSKGIKF
781EIFHKGSFFR SDKLVGTAHL KLERLENECE IREIVEVLDG RKPTGGKLEV KVRLREPLSG
841QDVQMVTENW LVLEPRGL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

858 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.