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CBX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CBX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G116119-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CBX2 (NM_005189), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Chromobox homolog 2 | Chromobox protein homolog 2 | M33 | SRXY5 | CBX2 | CDCA6 | Modifier 3 | Pc class homolog
Origin Species
Human
NCBI GenBank
NM_005189
SwissProt
Q14781

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEELSSVGEQ VFAAECILSK RLRKGKLEYL VKWRGWSSKH NSWEPEENIL DPRLLLAFQK
61KEHEKEVQNR KRGKRPRGRP RKLTAMSSCS RRSKLKEPDA PSKSKSSSSS SSSTSSSSSS
121DEEDDSDLDA KRGPRGRETH PVPQKKAQIL VAKPELKDPI RKKRGRKPLP PEQKATRRPV
181SLAKVLKTAR KDLGAPASKL PPPLSAPVAG LAALKAHAKE ACGGPSAMAT PENLASLMKG
241MASSPGRGGI SWQSSIVHYM NRMTQSQAQA ASRLALKAQA TNKCGLGLDL KVRTQKGELG
301MSPPGSKIPK APSGGAVEQK VGNTGGPPHT HGASRVPAGC PGPQPAPTQE LSLQVLDLQS
361VKNGMPGVGL LARHATATKG VPATNPAPGK GTGSGLIGAS GATMPTDTSK SEKLASRAVA
421PPTPASKRDC VKGSATPSGQ ESRTAPGEAR KAATLPEMSA GEESSSSDSD PDSASPPSTG
481QNPSVSVQTS QDWKPTRSLI EHVFVTDVTA NLITVTVKES PTSVGFFNLR HY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

532 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.