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CASS4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CASS4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G128357-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CASS4 (NM_001164116), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CASS4 | CAS4 | HEF-like protein | HEPL | HEF1-EFS-p130Cas-like protein | C20orf32 | HEFL
Origin Species
Human
NCBI GenBank
NM_020356
SwissProt
Q9NQ75

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKGTGIMDCA PKALLARALY DNCPDCSDEL AFSRGDILTI LEQHVPESEG WWKCLLHGRQ
61GLAPANRLQI LTEVAADRPC PPFLRGLEEA PASSEETYQV PTLPRPPTPG PVYEQMRSWA
121EGPQPPTAQV YEFPDPPTSA RIICEKTLSF PKQAILTLPR PVRASLPTLP SQVYDVPTQH
181RGPVVLKEPE KQQLYDIPAS PKKAGLHPPD SQASGQGVPL ISVTTLRRGG YSTLPNPQKS
241EWIYDTPVSP GKASVRNTPL TSFAEESRPH ALPSSSSTFY NPPSGRSRSL TPQLNNNVPM
301QKKLSLPEIP SYGFLVPRGT FPLDEDVSYK VPSSFLIPRV EQQNTKPNIY DIPKATSSVS
361QAGKELEKAK EVSENSAGHN SSWFSRRTTS PSPEPDRLSG SSSDSRASIV SSCSTTSTDD
421SSSSSSEESA KELSLDLDVA KETVMALQHK VVSSVAGLML FVSRKWRFRD YLEANIDAIH
481RSTDHIEESV REFLDFARGV HGTACNLTDS NLQNRIRDQM QTISNSYRIL LETKESLDNR
541NWPLEVLVTD SVQNSPDDLE RFVMVARMLP EDIKRFASIV IANGRLLFKR NCEKEETVQL
601TPNAEFKCEK YIQPPQRETE SHQKSTPSTK QREDEHSSEL LKKNRANICG QNPGPLIPQP
661SSQQTPERKP RLSEHCRLYF GALFKAISAF HGSLSSSQPA EIITQSKLVI MVGQKLVDTL
721CMETQERDVR NEILRGSSHL CSLLKDVALA TKNAVLTYPS PAALGHLQAE AEKLEQHTRQ
781FRGTLG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

786 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.