Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CASQ1 / Calsequestrin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118711-100
Catalog Number: LS-G118711
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CASQ1 (NM_001231), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CASQ | CASQ1 | Calsequestrin-1 | Calmitine | PDIB1 | Calmitin
- Origin Species
- Human
- NCBI GenBank
- NM_001231
- SwissProt
- P31415
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPRAVPGLR LALLLLLVLG TPKSGVQGQE GLDFPEYDGV DRVINVNAKN YKNVFKKYEV61LALLYHEPPE DDKASQRQFE MEELILELAA QVLEDKGVGF GLVDSEKDAA VAKKLGLTEV121DSMYVFKGDE VIEYDGEFSA DTIVEFLLDV LEDPVELIEG ERELQAFENI EDEIKLIGYF181KSKDSEHYKA FEDAAEEFHP YIPFFATFDS KVAKKLTLKL NEIDFYEAFM EEPVTIPDKP241NSEEEIVNFV EEHRRSTLRK LKPESMYETW EDDMDGIHIV AFAEEADPDG FEFLETLKAV301AQDNTENPDL SIIWIDPDDF PLLVPYWEKT FDIDLSAPQI GVVNVTDADS VWMEMDDEED361LPSAEELEDW LEDVLEGEIN TEDDDDDDDDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
390 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.