Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CASP8 / Caspase 8 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118713-100
Catalog Number: LS-G118713
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Caspase 8(CASP8) (NM_001228), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ALPS2B | Apoptotic protease Mch-5 | Apoptotic cysteine protease | Casp-8 | Caspase-8 | CAP4 | Caspase 8 | MACH | MCH5 | MORT1-associated ced-3 homolog | ICE-like apoptotic protease 5 | Pro Caspase 8 | CASP8 | FADD-like ICE | FLICE | MACH-alpha-1/2/3 protein | MACH-beta-1/2/3/4 protein
- Origin Species
- Human
- NCBI GenBank
- nm_001228
- SwissProt
- Q14790
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDFSRNLYDI GEQLDSEDLA SLKFLSLDYI PQRKQEPIKD ALMLFQRLQE KRMLEESNLS61FLKELLFRIN RLDLLITYLN TRKEEMEREL QTPGRAQISA YRFHFCRMSW AEANSQCQTQ121SVPFWRRVDH LLIRVMLYQI SEEVSRSELR SFKFLLQEEI SKCKLDDDMN LLDIFIEMEK181RVILGEGKLD ILKRVCAQIN KSLLKIINDY EEFSKGEELC GVMTISDSPR EQDSESQTLD241KVYQMKSKPR GYCLIINNHN FAKAREKVPK LHSIRDRNGT HLDAGALTTT FEELHFEIKP301HDDCTVEQIY EILKIYQLMD HSNMDCFICC ILSHGDKGII YGTDGQEAPI YELTSQFTGL361KCPSLAGKPK VFFIQACQGD NYQKGIPVET DSEEQPYLEM DLSSPQTRYI PDEADFLLGM421ATVNNCVSYR NPAEGTWYIQ SLCQSLRERC PRGDDILTIL TEVNYEVSNK DDKKNMGKQM481PQPTFTLRKK LVFPSDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
496 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.