Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CASP7 / Caspase 7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108289-100
Catalog Number: LS-G108289
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Caspase-7(CASP7) (NM_033338), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Apoptotic protease Mch-3 | CASP7 | Caspase 7 | Caspase-7 | ICE-LAP3 | LICE2 | MCH3 | ICE-like apoptotic protease 3 | CASP-7 | CMH-1
- Origin Species
- Human
- NCBI GenBank
- NM_001227
- SwissProt
- P55210
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDCVGWPPGR KWHLEKNTSC GGSSGICASY VTQMADDQGC IEEQGVEDSA NEDSVDAKPD61RSSFVPSLFS KKKKNVTMRS IKTTRDRVPT YQYNMNFEKL GKCIIINNKN FDKVTGMGVR121NGTDKDAEAL FKCFRSLGFD VIVYNDCSCA KMQDLLKKAS EEDHTNAACF ACILLSHGEE181NVIYGKDGVT PIKDLTAHFR GDRCKTLLEK PKLFFIQACR GTELDDGIQA DSGPINDTDA241NPRYKIPVEA DFLFAYSTVP GYYSWRSPGR GSWFVQALCS ILEEHGKDLE IMQILTRVND301RVARHFESQS DDPHFHEKKQ IPCVVSMLTK ELYFSQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
336 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.