Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CASP5 / Caspase 5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100270-100
Catalog Number: LS-G100270
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Caspase 5(CASP5) (NM_004347), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CASP-5 | CASP5 | Caspase 5 | Caspase-5 | ICE(rel)III | ICEREL-III | ICE(rel)-III | Ich-3 | Protease TY | ICH3 | Protease ICH-3 | TY protease
- Origin Species
- Hu
- NCBI GenBank
- NM_004347
- SwissProt
- P51878
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 47.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MFKGILQSGL DNFVINHMLK NNVAGQTSIQ TLVPNTDQKS TSVKKDNHKK KTVKMLEYLG61KDVLHGVFNY LAKHDVLTLK EEEKKKYYDA KIEDKALILV DSLRKNRVAH QMFTQTLLNM121DQKITSVKPL LQIEAGPPES AESTNILKLC PREEFLRLCK KNHDEIYPIK KREDRRRLAL181IICNTKFDHL PARNGAHYDI VGMKRLLQGL GYTVVDEKNL TARDMESVLR AFAARPEHKS241SDSTFLVLMS HGILEGICGT AHKKKKPDVL LYDTIFQIFN NRNCLSLKDK PKVIIVQACR301GEKHGELWVR DSPASLALIS SQSSENLEAD SVCKIHEEKD FIAFCSSTPH NVSWRDRTRG361SIFITELITC FQKYSCCCHL MEIFRKVQKS FEVPQAKAQM PTIERATLTR DFYLFPGNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
418 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.