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CASKIN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CASKIN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G126063-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CASKIN2 (NM_001142643), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKS5B | CASK interacting protein 2 | Caskin-2 | CASKIN2 | KIAA1139
Origin Species
Human
NCBI GenBank
AB032965
SwissProt
Q8WXE0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
118.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRPLHYAAWQ GRLEPVRLLL RASAAVNAAS LDGQIPLHLA AQYGHYEVSE MLLQHQSNPC
61LVNKAKKTPL DLACEFGRLK VAQLLLNSHL CVALLEGEAK DPCDPNYTTP LHLAAKNGHR
121EVIRQLLRAG IEINRQTKTG TALHEAALYG KTEVVRLLLE GGVDVNIRNT YNQTALDIVN
181QFTTSQASRE IKQLLREASG ILKVRALKDF WNLHDPTALN VRAGDVITVL EQHPDGRWKG
241HIHESQRGTD RIGYFPPGIV EVVSKRVGIP AARLPSAPTP LRPGFSRTPQ PPAEEPPHPL
301TYSQLPRVGL SPDSPAGDRN SVGSEGSVGS IRSAGSGQSS EGTNGHGPGL LIENAQPLPS
361AGEDQVLPGL HPPSLADNLS HRPLANCRSG EQIFTQDVRP EQLLEGKDAQ AIHNWLSEFQ
421LEGYTAHFLQ AGYDVPTISR MTPEDLTAIG VTKPGHRKKI ASEIAQLSIA EWLPSYIPTD
481LLEWLCALGL PQYHKQLVSS GYDSMGLVAD LTWEELQEIG VNKLGHQKKL MLGVKRLAEL
541RRGLLQGEAL SEGGRRLAKG PELMAIEGLE NGEGPATAGP RLLTFQGSEL SPELQAAMAG
601GGPEPLPLPP ARSPSQESIG ARSRGSGHSQ EQPAPQPSGG DPSPPQERNL PEGTERPPKL
661CSSLPGQGPP PYVFMYPQGS PSSPAPGPPP GAPWAFSYLA GPPATPPDPP RPKRRSHSLS
721RPGPTEGDAE GEAEGPVGST LGSYATLTRR PGRSALVRTS PSVTPTPARG TPRSQSFALR
781ARRKGPPPPP PKRLSSVSGP SPEPPPLDES PGPKEGATGP RRRTLSEPAG PSEPPGPPAP
841AGPASDTEEE EPGPEGTPPS RGSSGEGLPF AEEGNLTIKQ RPKPAGPPPR ETPVPPGLDF
901NLTESDTVKR RPKCREREPL QTALLAFGVA SATPGPAAPL PSPTPGESPP ASSLPQPEPS
961SLPAQGVPTP LAPSPAMQPP VPPCPGPGLE SSAASRWNGE TEPPAAPAAL LKVPGAGTAP
1021KPVSVACTQL AFSGPKLAPR LGPRPVPPPR PESTGTVGPG QAQQRLEQTS SSLAAALRAA
1081EKSIGTKEQE GTPSASTKHI LDDISTMFDA LADQLDAMLD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1120 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.