Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CARS1 / CARS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121575-100
Catalog Number: LS-G121575
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CARS (NM_001014437), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CARS | Cysteinyl-tRNA synthetase | CYSRS | Cysteine translase | CARS1 | MGC:11246
- Origin Species
- Human
- NCBI GenBank
- NM_001751
- SwissProt
- P49589
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 94.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADSSGQQAP DYRSILSISD EAARAQALNE HLSTRSYVQG YSLSQADVDA FRQLSAPPAD61PQLFHVARWF RHIEALLGSP CGKGQPCRLQ ASKGRRVQPQ WSPPAGTQPC RLHLYNSLTR121NKEVFIPQDG KKVTWYCCGP TVYDASHMGH ARSYISFDIL RRVLKDYFKF DVFYCMNITD181IDDKIIKRAR QNHLFEQYRE KRPEAAQLLE DVQAALKPFS VKLNETTDPD KKQMLERIQH241AVQLATEPLE KAVQSRLTGE EVNSCVEVLL EEAKDLLSDW LDSTLGCDVT DNSIFSKLPK301FWEGDFHRDM EALNVLPPDV LTRVSEYVPE IVNFVQKIVD NGYGYVSNGS VYFDTAKFAS361SEKHSYGKLV PEAVGDQKAL QEGEGDLSIS ADRLSEKRSP NDFALWKASK PGEPSWPCPW421GKGRPGWHIE CSAMAGTLLG ASMDIHGGGF DLRFPHHDNE LAQSEAYFEN DCWVRYFLHT481GHLTIAGCKM SKSLKNFITI KDALKKHSAR QLRLAFLMHS WKDTLDYSSN TMESALQYEK541FLNEFFLNVK DILRAPVDIT GQFEKWGEEE AELNKNFYDK KTAIHKALCD NVDTRTVMEE601MRALVSQCNL YMAARKAVRK RPNQALLENI ALYLTHMLKI FGAVEEDSSL GFPVGGPGTS661LSLEATVMPY LQVLSEFREG VRKIAREQKV PEILQLSDAL RDNILPELGV RFEDHEGLPT721VVKLVDRNTL LKEREEKRRV EEEKRKKKEE AARRKQEQEA AKLAKMKIPP SEMFLSETDK781YSKFDENGLP THDMEGKELS KGQAKKLKKL FEAQEKLYKE YLQMAQNGSF QSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
831 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.