Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Carom / FCHSD2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113696-100
Catalog Number: LS-G113696
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FCHSD2 (NM_014824), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Carom | FCH and double SH3 domains 2 | FCHSD2 | NWK | KIAA0769 | Nervous wreck homolog | SH3 multiple domains 3 | SH3 multiple domains protein 3 | SH3MD3
- Origin Species
- Human
- SwissProt
- O94868
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MQKLASQYLK RDWPGVKADD RNDYRSMYPV WKSFLEGTMQ VAQSRMNICE NYKNFISEPA61RTVRSLKEQQ LKRCVDQLTK IQTELQETVK DLAKGKKKYF ETEQMAHAVR EKADIEAKSK121LSLFQSRISL QKASVKLKAR RSECNSKATH ARNDYLLTLA AANAHQDRYY QTDLVNIMKA181LDGNVYDHLK DYLIAFSRTE LETCQAVQNT FQFLLENSSK VVRDYNLQLF LQENAVFHKP241QPFQFQPCDS DTSRQLESET GTTEEHSLNK EARKWATRVA REHKNIVHQQ RVLNDLECHG301AAVSEQSRAE LEQKIDEARE NIRKAEIIKL KAEARLDLLK QIGVSVDTWL KSAMNQVMEE361LENERWARPP AVTSNGTLHS LNADTEREEG EEFEDNMDVF DDSSSSPSGT LRNYPLTCKV421VYSYKASQPD ELTIEEHEVL EVIEDGDMED WVKARNKVGQ VGYVPEKYLQ FPTSNSLLSM481LQSLAALDSR SHTSSNSTEA ELVSGSLNGD ASVCFVKALY DYEGQTDDEL SFPEGAIIRI541LNKENQDDDG FWEGEFNGRI GVFPSVLVEE LSASENGDTP WMREIQISPS PKPHASLPPL601PLYDQPPSSP YPSPDKRSSL YFPRSPSANE KSLHAESPGF SQASRHTPET SYGKLRPVRA661APPPPTQNHR RPAEKIEDVE ITLVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
684 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.