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CARNS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CARNS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CARNS1 antibodies →
$725.00each
Catalog Number: LS-G128399-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CARNS1 (NM_001166222), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATP-grasp domain containing 1 | KIAA1394 | ATPGD1 | Carnosine synthase 1 | CARNS1
Origin Species
Human
NCBI GenBank
NM_001166222
SwissProt
A5YM72

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLSLDPSGPE WDCPLGSKDL EEEGPWGGGS GLPPTGCFPG SWRQDVGLDC KGSPEGAEAR
61AWTVYYYSLL QSCLQQAGLP ETQDRGQVPR TGCPGAEVTL CVLGSPSTFL PVLLEGGVQS
121PGNMLLCLSP AWLMKVPAPG QPGEAALLVS KAVSFHPGGL TFLDDFVPPR RATYFLAGLG
181LGPGRGREAA ELARDLTCPT GASAELARLL EDRLLTRQLL AQQGGVAVPA TLAFTYKPPG
241LLRGGDASLG LRLVELSGKE GQETLVKEEV EAFLRSEALG DILQVAVKLS GWRWRGRQAW
301RLHPRAELGA VVDTVLALLE KLEEEESVLV EAVYPPAQLP CSDGPSPGPG LAVRICAVVC
361RTQGDRPLLS KVVCGVGRGD RPLRHHNSLP RTLEVALAQC GLGEEAQVAA VRQRVKAAAE
421AALAAVLALE AGLSAEQRGG RRAHTDFLGV DFALTAAGGV LTPVALELNG GLCLEACGAL
481EGLWAAPRLG PAADEAVAAP LVETMLRRSA RCLMEGKQLL VVGAGGVSKK FVWEAARDYG
541LQLHLVESDP NHFASQLVQT FIHFDMTEHR RDEENARLLA ELVRARGLKL DGCFSYWDDC
601LVLTALLCQE LGLPCSSPAA MRLAKQKSLT QLHLLHHHGP PWPAPSLHAV PCCPLESEAD
661VERAVHQVPL PGVMKLEFGA GAVGVRLVED APQCHEHFSR ITRDLQGEAD HPGIGLGWGN
721AMLLMEFVEG TEHDVDLVLF GGRLLAAFVS DNGPTRLPGF TETAACMPTG LAPEQEAQMV
781QAAFRCCLGC GLLDGVFNVE LKLTGAGPRL IEINPRMGGF YLRDWILELY GVDLLLAAVM
841VACGLRPALP TRPRARGHLV GVMCLVSQHL QALSSTASRE TLQALHDRGL LRLNLLEEAL
901VPGEYEEPYC SVACAGPSPT EARLRLLGLC QGLGIDGPSY PVAHFLSHFK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

950 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.