Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CARD10 / CARMA3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113893-100
Catalog Number: LS-G113893
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CARD10 (NM_014550), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CARMA3 | BIMP1 | CARD10 | Carma 3
- Origin Species
- Human
- NCBI GenBank
- NM_014550
- SwissProt
- Q9BWT7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 115.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPGRAEAGEA EEEAGAGSGS EAEEDALWER IEGVRHRLAR ALNPAKLTPY LRQCRVIDEQ61DEEEVLSTYR FPCRVNRTGR LMDILRCRGK RGYEAFLEAL EFYYPEHFTL LTGQEPAQRC121SMILDEEGPE GLTQFLMTEV RRLREARKSQ LQREQQLQAR GRVLEEERAG LEQRLRDQQQ181AQERCQRLRE DWEAGSLELL RLKDENYMIA MRLAQLSEEK NSAVLRSRDL QLAVDQLKLK241VSRLEEECAL LRRARGPPPG AEEKEKEKEK EKEPDNVDLV SELRAENQRL TASLRELQEG301LQQEASRPGA PGSERILLDI LEHDWREAQD SRQELCQKLH AVQGELQWAE ELRDQYLQEM361EDLRLKHRTL QKDCDLYKHR MATVLAQLEE IEKERDQAIQ SRDRIQLQYS QSLIEKDQYR421KQVRGLEAER DELLTTLTSL EGTKALLEVQ LQRAQGGTCL KACASSHSLC SNLSSTWSLS481EFPSPLGGPE ATGEAAVMGG PEPHNSEEAT DSEKEINRLS ILPFPPSAGS ILRRQREEDP541APPKRSFSSM SDITGSVTLK PWSPGLSSSS SSDSVWPLGK PEGLLARGCG LDFLNRSLAI601RVSGRSPPGG PEPQDKGPDG LSFYGDRWSG AVVRRVLSGP GSARMEPREQ RVEAAGLEGA661CLEAEAQQRT LLWNQGSTLP SLMDSKACQS FHEALEAWAK GPGAEPFYIR ANLTLPERAD721PHALCVKAQE ILRLVDSAYK RRQEWFCTRV DPLTLRDLDR GTVPNYQRAQ QLLEVQEKCL781PSSRHRGPRS NLKKRALDQL RLVRPKPVGA PAGDSPDQLL LEPCAEPERS LRPYSLVRPL841LVSALRPVVL LPECLAPRLI RNLLDLPSSR LDFQVCPAES LSGEELCPSS APGAPKAQPA901TPGLGSRIRA IQESVGKKHC LLELGARGVR ELVQNEIYPI VIHVEVTEKN VREVRGLLGR961PGWRDSELLR QCRGSEQVLW GLPCSWVQVP AHEWGHAEEL AKVVRGRILQ EQARLVWVEC1021GSSRGCPSSS EATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1032 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.