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Carboxylesterase 5A / CES5A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Carboxylesterase 5A / CES5A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CES5A antibodies →
$504.00each
Catalog Number: LS-G106799-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Carboxylesterase 7(CES5A) (NM_145024), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Carboxylesterase 5A | CES4C1 | CES5 | CES5A | CES7 | Carboxylesterase 5 | Carboxylesterase 7 | CAUXIN
Origin Species
Human
NCBI GenBank
NM_001143685
SwissProt
Q6NT32

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSGNWVHPGQ ILIWAIWVLA APTKGPSAEG PQRNTRLGWI QGKQVTVLGS PVPVNVFLGV
61PFAAPPLGSL RFTNPQPASP WDNLREATSY PNLCLQNSEW LLLDQHMLKV HYPKFGVSED
121CLYLNIYAPA HADTGSKLPV LVWFPGGAFK TGSASIFDGS ALAAYEDVLV VVVQYRLGIF
181GFFTTWDQHA PGNWAFKDQV AALSWVQKNI EFFGGDPSSV TIFGESAGAI SVSSLILSPM
241AKGLFHKAIM ESGVAIIPYL EAHDYEKSED LQVVAHFCGN NASDSEALLR CLRTKPSKEL
301LTLSQKTKSF TRVVDGAFFP NEPLDLLSQK AFKAIPSIIG VNNHECGFLL PMKEAPEILS
361GSNKSLALHL IQNILHIPPQ YLHLVANEYF HDKHSLTEIR DSLLDLLGDV FFVVPALITA
421RYHREGATEE EKLLSRKMMK YWATFARTGN PNGNDLSLWP AYNLTEQYLQ LDLNMSLGQR
481LKEPRVDFWT STIPLILSAS DMLHSPLSSL TFLSLLQPFF FFCAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

525 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.